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To compare the relative amounts of protein and phosphorylation across brain regions, we prepared PSD samples from microdissections of cerebellum, cortex, hippocampus, and midbrain. A small fraction of α7 puncta was also seen at filopodia-like structures (A, C, arrows) that usually do not have presynaptic counterparts (D). Matured mushroom-like spines are marked by arrowheads.
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